Southern blotting can also be used to identify methylated sites in particular genes.
2.
Bulked DNA samples can be analysed using Southern blotting.
3.
This process is termed Southern blotting.
4.
DNA microarrays evolved from Southern blotting which allows for detection of a specific DNA sequence in a sample of DNA.
5.
In hereditary VHL, disease techniques such as southern blotting and gene sequencing can be used to analyse DNA and identify mutations.
6.
Using Southern blotting and fluorescence in situ hybridization ( FISH ) on the genome, the researchers found no evidence of DNA rearrangement.
7.
The use of DNA polymerase to extend oligonucleotide primers was a common procedure in DNA sequencing and the production of DNA probes for Southern blotting.
8.
Southern blotting is less commonly used in laboratory science due to the capacity of other techniques, such as PCR, to detect specific DNA sequences from DNA samples.
9.
Its use is not limited to molecular cloning; it is also used in restriction site mapping, genotyping, southern blotting, restriction fragment length polymorphism ( RFLP ) and SNP.
10.
The name " southwestern blotting " is based on the fact that this technique detects DNA-binding proteins, since DNA detection is by Southern blotting and protein detection is by western blotting.
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